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An investigation of small mammal distribution and their infection with pathogens in rural areas of Quzhou, Zhejiang province, China
ZHONG Jian-yue, WANG Jin-na, YANG Hui, CAO Guo-ping, WU Yu-yan, CHEN Wei-hua, YU Zhang-you, FANG Chun-fu
Abstract155)      PDF (915KB)(753)      
Objective To investigate the small mammal distribution and their infection with pathogens in rural areas of Quzhou, Zhejiang province, China, and to provide a scientific basis for the prevention and control of rodent-borne diseases in Quzhou. Methods From September 2020 to July 2021, the night snap trapping method was used to monitor small mammal species in Kecheng district and Longyou county of Quzhou. The viscera of small mammals, including liver, spleen, kidney, and lung, were collected. Quantitative real-time PCR was used to detect the nucleic acids of severe fever with thrombocytopenia syndrome virus (SFTSV), Orientia tsutsugamushi (Ot), Leptospira, and Hantavirus. Results A total of 210 small mammals were captured during the survey of Quzhou monitoring sites, with a capture rate of 8.44%. March, September, and November were the peak months of small mammal infestation. Apodemus agrarius (130/210, 61.91%) was the dominant species in rural areas of Quzhou, followed by Rattus tanezumi (32/210, 15.24%) and R. norvegicus (20/210, 9.52%). In terms of various habitats, R. tanezumi and R. norvegicus were the dominant species in rural residential areas, and A. agrarius was the dominant species in farmland and forest land. A total of 210 visceral specimens were collected from the small mammals, of which 13 (6.19%) were positive for hantavirus nucleic acid, 7 (3.33%) were positive for Leptospira nucleic acid, and SFTSV and Ot were not detected. There was a statistical difference between the detection rates of 4 pathogens ( χ 2=24.176, P<0.001). In terms of various small mammal species, hantavirus nucleic acid was detected only from A. agrarius, with a positive rate of 10.00% (13/130); Leptospira nucleic acid was detected from R. norvegicus, A. agrarius, and R. tanezumi, with positive rates of 5.00% (1/20), 3.85% (5/130), and 3.13% (1/32), respectively; there was no statistical difference between the positive rates ( χ 2=0.114, P=0.945). Some A. agrarius rodents were co-infected with hantavirus and Leptospira. Conclusion A. agrarius, R. tanezumi, and R. norvegicus are the dominant species in rural areas of Quzhou. A. agrarius carries hantavirus and Leptospira; R. tanezumi and R. norvegicus carry Leptospira. It is necessary to strengthen the publicity of prevention and control of rodent-borne diseases and carry out patriotic health campaigns of rodent control and disease prevention to reduce the risk of infection with rodent-borne diseases.
2022, 33 (6): 805-808.   doi: 10.11853/j.issn.1003.8280.2022.06.007
Epidemiological characteristics of hemorrhagic fever with renal syndrome and surveillance studies of host animal in Quzhou, Zhejiang province, China, 2006-2020
WANG Min, YANG Hui, YU Zhang-you, ZHONG Jian-yue, FANG Chun-fu, WU Shi-quan, GONG Zhen-yu
Abstract239)      PDF (951KB)(732)      
Objective To investigate the epidemiological characteristics of hemorrhagic fever with renal syndrome (HFRS) as well as the species composition and Hantavirus infection status of host animals in Quzhou, Zhejiang province, China, and to provide a reference for formulating scientific prevention and control measures. Methods The incidence data of HFRS in Quzhou from 2006 to 2020 were analyzed. The night snap-trapping method was used to capture small mammals to calculate the capture rate and analyze the species composition of small mammals. The lungs and blood of the small mammals were collected to detect Hantavirus antigen and antibody for information on hantavirus infection among the small mammals. The Chi-squared test was used to compare the rates. Results A total of 720 cases of HFRS were reported in Quzhou from 2006 to 2020, with an average annual incidence rate of 2.13/100 000. Of those, there were two deaths, with a fatality rate of 0.28%. The cases were mainly aged between 30 and 69 years, accounting for 92.08% of total cases. Farmers accounted for 80.14% of total cases. The male-to-female ratio was 2.35:1. The incidence of HFRS peaked during May to July and October to next January. The average annual incidence was highest in Kaihua county, which was 8.81/100 000. The indoor and outdoor capture rates of small mammals were 5.57% and 6.14%, respectively, showing a statistical difference ( χ 2=7.374, P=0.007). Rattus norvegicus was the dominant species indoors, accounting for 41.18%, and Apodemus agrarius was the dominant species outdoors, accounting for 62.97%. There was a statistical difference in the species composition of small mammals indoors and outdoors ( χ 2=1 343.773, P<0.001). The antigen-positive rate of Hantavirus averaged 2.42%, and differed statistically between different species of small mammals ( χ 2=17.260, P=0.004). The serum antibody-positive rate for Hantavirus averaged 6.71%, and differed statistically between different species of small mammals ( χ 2=32.923, P<0.001). Conclusion There is a high density of small mammals and a high infection rate of Hantavirus among these small mammals in Quzhou. Cases of HFRS are mainly middle-aged and elderly, with high proportions of males and farmers. Its incidence shows double peaks in summer and winter. It is still necessary to strengthen comprehensive control measures combining HFRS monitoring, rodent control, health education, and vaccination for prevention and control of HFRS.
2022, 33 (4): 480-484.   doi: 10.11853/j.issn.1003.8280.2022.04.007
Investigation on Borrelia burgdorferi in rats captured at Kaihua county in Quzhou of Zhejiang province
WANG Shuang-qing, ZHAN Bing-dong, ZHANG Jian-min, CAO Guo-ping, ZHONG Jian-yue, YU Zhang-you
Abstract257)      PDF (635KB)(839)      
Objective The present study was conducted to investigate the infection of Borrelia burgdorferi in rodents in Kaihua county. Methods Nested PCR were used to amplify 5S-23S rRNA spacer fragments of B. burgdorferi from kidney samples of rodents captured by mouse cage in Kaihua county. And the positive samples were sequenced and analyzed. Results A total of 10 rodents were positive in 149 samples tested,with the prevalence of 6.71%. The positive samples were identified from Rattus edwardsi, Niviventer niviventer, N. fulvescens, R. tanezumi, Apodemus agrarius, and R. norvegicus. The predominant species in Kaihua county were Ap. agrarius, R. fulvescens, and N. niviventer and the positive rates were 4.05%, 10.00%, and 10.00%, respectively. The spleen specimens from the N. confucianus and N. fulvescens were positive for B.burgdorferi sensu stricto, and that from R. norvegicus was positive for B.valaisiana. Conclusion The N. confucianus and N. fulvescens are considered as important reservoirs. The infection of two B. burgdorferi genotypes in rodents was confirmed in Kaihua county. It is very important to enhance the B. burgdorferi surveillance in wild animals and focus the morbidity in population.
2018, 29 (6): 601-603.    doi: 10.11853/j.issn.1003.8280.2018.06.012
Early warning and surveillance of plague in historical epidemic areas of Quzhou, Zhejiang province, China during 2004-2012
YU Zhang-you
Abstract391)      PDF (803KB)(768)      

Objective To monitor plague host animals, its vectors and plague epidemic and to provide a scientific basis for timely detection, prevention and control of plague epidemic. Methods Live rats were captured with cage traps in the daytime and were then taxonomically identified. The liver, spleen, and serum samples were collected from the rats for isolation of plague bacteria and detection of F1 antibody. The animals with unexplained death were searched for, and epidemic surveillance was conducted. Results TThe dominant species of house rats were Rattus norvegicus and R. tanezumi, and the dominant species of field rats was Apodemus agrarius. The mean rodent densities were 3.19% indoor and 2.82% outdoors. The mean flea-carrying rate of rodents was 1.89%, and the total flea index of rodents was 0.0493. The dominant species of rat fleas was Leptopsylla segnis. The samples from live rats were negative for F1 antibody and pathogens. Plague cases were not found althouth large numbers of animals with unexplained death. Conclusion The species compositions of plague hosts and vectors in historical epidemic areas remain stable with low densities, and plague epidemic has not been found. However, plague surveillance should still be performed for timely detection of abnormal signals and prevention of human cases.

2013, 24 (5): 453-456.    doi: 10.11853/j.issn.1003.4692.2013.05.022
Density surveillance and effect of control measures on mosquitoes and flies in Quzhou city
YU Zhang-you, WANG Xiao-lin, HU Xiao-long
Abstract1410)      PDF (918KB)(878)      

Objective To evaluate effect of control measures on mosquito and fly populationsin urban Quzhou based on the monitoring of their species and density. Methods The mosquito and fly density was monitored using lamp traps and cage traps, respectively. Results There were 4 species and 3 genera of mosquitoes in urban areas, predominated by Culex pipiens pallens, which accounted for 96.82% of the total population. The anual average adult density was 5.28 per lamp-hour, which peaked from June to August. There were 6 genera and 4 families of flies in this area, predominated by Lucilia and Musca, which accounted for 68.22% and 23.82%, respectively. The annual average adult density was 39.74 per cage, which peaked from June to August. Conclusion As a result of comprehensive control strategies, diminishing breeding grounds in the city have lead to a significant decrease in mosquito and fly population densities.

2011, 22 (4): 380-382.
Epidemic situation and investigation of surveillance on rabies in Quzhou city from 1985 to 2007
YIN Zhi-Ying, YU Zhang-You, CHEN Yi, LI Jun-Ji
Abstract1423)      PDF (373KB)(922)      

【Abstract】 Objective To analyze the epidemic characteristics of rabies from 1985 to 2007, and explore the reasons causing the increase of rabies and to provide the control measures. Methods The data of rabies incidences collected from 1985-2007 were classified and analyzed by Excel 2003 and SPSS 13.0. Results There were 31 cases of rabies during 1985-2007 in Quzhou, and 31 died, the mortality was 100%. Most of the cases occurred before 1992, and there were no case from 1993 to 2003.But the incidence gradually increased after 2004. There were cases reported in all counties except for Kaihua, and the epidemic could be seen each month. Children and young adults were more frequently affected and the incidence in males was higher than that in females. The peasants were the most susceptible peoples, which accounted for 77.42%. The cases were caused by dog bitten and most of them did not receive wound treatment or immunization. In recent years, the exposure rate of people increased from year to year, but the immunization rate after exposed tended to reduce. Conclusion The incidence of rabies in Quzhou presented a rapid rising trend. The increase of dog quantities leaded to the rising of exposure rate. The reasons leading to the increase of epidemic situation were the shortage of disease prevention knowledge, non?timely treatment of exposure wounds and immunization and the rising of rabies vaccine price. It should take specific measures to control rabies.

2009, 20 (5): 464-467.
Study on the surveillance of leptospirosis in Quzhou from 2001 to 2005
FANG Chun-fu; YU Zhang-you; WANG Wei; WANG Chui-zhang; LEI Jin-bao; DUAN Jia-fu; MAO Hui-shu
Abstract1184)      PDF (379KB)(873)      
Objective To study the epidemiological features and the situation of Quzhou leptospirosis foci, and provide the control measures of leptospirosis outbreak. Methods Analyze the leptospirosis epidemic situation of 1960-2005 by descriptive epidemiological method in Quzhou. In order to determine the main serogroup of leptospirosis carried by the host animals, we collected kidney samples of rats, teals, pigs, frogs and the serum of teals and frogs as well as the midstream urine of cattle. All samples were used to cultivate the pathogen and test the antibody. Results 11 047 cases were reported in Quzhou from 1960-2005, and the annual incidence was 11.58/100 000. Of which, 72 cases died and the mortality was 0.65%. The cases from July to September accounted for 90.26% of the total, and the farmer patients accounted for 88.20%. The proportion of male to female was 2.74∶1. The Changshan and Kaihua county were the main epidemic area. From 2001-2005, the rat density was 3.93% in the open air, and apodemus agrarius accounted for 51.40% of the total. The positive rate of samples was 0.49% and that of serum antibody was 14.02%. The pathogen of patients serum were mainly hebdomadis and icterohemorrhage, while that of host animals were icterohemorrhage and autumnal fever. Conclusion The leptospirosis incidence mainly affected by local outbreak. The peak of incidence was in summer and autumn, and the high-risk group was the man aged from 20 to 50 years old, especially the peasants. In the recent years, the incidence remained low and the carrier rate of host animals was also low, but because many host animals could carry much bacteria, the risk of outbreak still exist.
Analysis on Surveillance of Hemorrhagic Fever with Renal Syndrome in Quzhou City
FANG Chun-fu; YU Zhang-you; WANG Chui-zhang; WANG Wei; GAO Yi; LEI Lin-hai; WANG Xin-min
Abstract1382)      PDF (448KB)(753)      
Objective To study the epidemic characteristics,determine the type of epidemic area,and make the preventive strategy of hemorrhagic fever with renal syndrome(HFRS). Methods Descriptive epidemiology methods and epidemic area survey were applied,epidemic situation among humans and the main host animals were analyzed. Samples were collected and fluorescent antibody(FA) was used to detect HV antigen,and immunofluoresce assay(IFA) for antibody detection. Results Since 1965,a total of 18 422 cases occurred in Quzhou city,the annual average incidence rate was 21.87/100 000. Besides,271 cases died,and the mortality was 1.47%. The prevalence seasons changed obviously in different years. The ratio of cases occurring in winter to that in spring was 7.22∶1 before 1980s' then decreased to 1.18∶1 after 2000. Farmer accounted for 82.41% of all cases and the ratio of male to female was 2.20∶1,25-54 years old cases accounted for 78.26%. The Kaihua and Qujiang district were the main epidemic zones in Quzhou city. From 2002-2004,the rat density in the field was 3.64%,whereas that of indoor was 4.20%. Rattus norvegicus,Apodemus agrarius were the predominant species,with the indice of two species of rats with virus was 0.026,0.034 respectively. During the three years,the indice of mixed species of rats were 0.048,0.036,0.032 respectively,correlating with incidence rate. Conclusion During the last 40 years,since the first case reported in 1965,the incidence rate showed the typical single-peak mode,which seems prevalence of HFRS follows the laws of sporadic occurring,prevailing,the dying out. Quzhou city is still a Apodemus-type predominant epidemic area. For these years,the HFRS morbidity reduced not only because of the decreasing chances of people contacting virus,but also the decreasing indexes of rat with virus. We still should further strengthen prevention of the HFRS.